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A spore is a dormant form of a bacterium that allows it to survive in drastic environmental conditions. Spores have a tough external cover made of protein keratin that is resistant to heat and chemicals. Keratin also resists staining, so extreme measures are needed to stain the spore. Spore staining is performed and the characteristics of the organism are observed under the microscope with the use of the oil immersion lens.
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Malachite green is soluble in water and has low affinity for cellular material, so vegetative cells can be discolored with water. Vegetative cells are then countered by safranin Spores may be located in the center of the cell, at the end or between the end and middle of the cell.
Spore shape may also be used for diagnostic purposes.
Spores might be spherical or elliptic. Corynebacterium members may exhibit club- shaped swellings that may be confused with spores.
Spore staining differs between real spores and those structures. Air dry it and fix with gentle heat. Counterstain with 0.
Wash with water, dry and observe under oil immersion lens. Download this Article in PDF.
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Principle of Endospore Staining
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Endospore stain procedure
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